Journal of the Korean Physical Society

pISSN 0374-4884 eISSN 1976-8524
Qrcode

Article

J. Korean Phy. Soc. 2011; 59(2(2)): 709-712

Published online August 12, 2011     https://doi.org/10.3938/jkps.59.709

Copyright © The Korean Physical Society.

Suppression of Metastatic Potential in Human Colorectal Adenocarcinoma Cells Irradiated with Proton Beam

Kyung-Soo Nam, Yun-Hee Shon

Abstract

The use of a proton beam in radiation therapy has been producing promising clinical results worldwide. Metastasis is the primary cause of cancer mortality and is a major issue in successful cancer treatment. The purpose of this study was to investigate the effect of proton beam irradiation on the metastatic potential in human colorectal adenocarcinoma HT-29 cells by measuring the motility and the invasion, as well as the amount and activity of matrix metalloproteinase (MMP)-2 in vitro. Treatment of HT-29 cells with increasing doses of proton irradiation led to a dose-dependent decrease in cell migration. The invasiveness of HT-29 cells through a matrigel-coated membrane was also inhibited in a dose-dependent manner in a dose range of 0.5 ¡­ 32 Gy. The amounts of secreted MMP-2 decreased with increasing proton beam irradiation. The activity of MMP-2 in HT-29 cells was also inhibited by proton irradiation. Our data indicated that the metastatic potential in human colorectal adenocarcinoma HT-29 cells was suppressed by proton beam irradiation by inhibiting the motility and the invasion and by decreasing the amount and activity of MMP-2.

Keywords: Proton beam, Colon cancer, Metastasis, Invasion, Matrix metalloproteinase,